• Description

    Description

    BBG liderated by Dr Otero investigates  the application of biocatalysts to develop selective processes of synthesis of chemical products of application in different areas: functional foods, nutraceuticals, sustainable chemistry, biomedicine, phamacy, cosmetics, biodiesel, fine chemicals, etc.

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    The research Group of Biocatalysis and Bioenergy (BBG) belongs to the Institute of Catalysis and Petrochemistry, which is part of the Spanish Council for Scientific Research (CSIC), The Institute is one of the research centres included in the field of Chemistry and Chemical Technologies.
    The group liderated by Dr Otero investigates the application of biocatalysts to develop selective industrial processes in different areas: functional foods, nutraceuticals, sustainable chemistry, biomedicine, pharmaceutical, cosmetics, biodiesel, fine chemicals, etc.

    For the industrial development of new selective biocatalytic processes, BBG investigates immobilized enzymes working in both batch and continuous reactors. Since 1987, the group exploits the  advantages of lipase-catalysed bioprocesses in water and in the presence of organic solvents in the reaction media (selectivity, especificity, etc). BBG investigates the synthesis of compounds used as food additives or emulsifiers: mono-, di- and triacylglycerides, and fatty acid esters of monosaccharides, polyols, amines, alpha-hydroxyacids, biodiesel and phospholipids. The group has wide experience in most of the techniques that are relevant in the field of Biocatalysis: Methodology for the immobilization/stabilization of enzymes, reaction and reactor engineering. The chemical characterization of products and the progress of the enzymatic reactions are followed by TLC, HPLC, gas-liquid
    chromatography, NMR and others.

    The laboratory of BBG is integrated in the network of laboratories of Madrid+D, running analysis of FAME, glycerides, biodiesel according to directives EN ISO 9001. Analytical methods mostly used in characterization of food-grade fats and oils, and biodiesel are certified by AENOR (European National Agency of Normalization). Determination of FAMEs, oxidative stability, oil composition in terms of mono-, diand triacylglycerol, free fatty acids, water content, density, viscosity, solid fat content, etc are carried out.

    BBG group has established fruitful collaborations with the Dept. Chem. Eng. of Wisconsin Univ., the Technological Institute of Veracruz (Mexico) and Korea University, and has participated in different international programs (NATO, EuropeanUnion, Royal Soc. Of UK, Korean KOSEF, Romanian Academy of Sciences, etc). BBG liderates nacional and international scientific consortia for evaluation and demonstrationof functional properties of new oil formulations for patients with obesity, methabolicsyndrome, diabetes, colesterol, hipertension and cancer, and for pregnants, lactants andold citizens.

    BBG has developed a process of synthesis of biodiesel from sunflower oil. The processoptimization involves the design of new catalysts more efficient in the alcoholysisreaction, the reactor design and determination of optimal operational conditions. BBGalso organizes monographic courses about synthesis and characterization of biodiesel,methods of characterization of materials, enzymes, biocatalysis, biotransformations and lipids.

    Research Lines of BBG:

    *      Enzymatic preparation of fats free of trans fatty acids: margarines and others

    *      Synthesis and characterization of functional lipids and phospholípids, lipids rich or not in  polyunsaturated fatty acids or PUFA (omega-3, omega-6, CLA, etc)

    *      Synthesis and characterization of biodiesel from plant oils, algae, etc

    *      Synthesis and characterization of emulsifiers derived from amines, monosacharides, and sorbitol

    *      Synthesis of a-hydroxyacids, oligosaccharides and others

    *      Identification and extraction of biomass subproducts (antioxidants, vitamins, phospholipids, antimicrobials, glycerides, fatty acids, etc)

    *      Characterization of fats, oils and biodiesel according with European directives:

    -Chemical characterization (HPLC-mass, CG-mass, etc for FAME, TAG, DAG, MAG, G, free fatty acids, humidity by Karl Fisher, etc)

    -Physical and functional characterization (meeting and crystallization profiles of fats, oxidative stability (Rancimat), viscosity, density, etc

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    Facilities & Technologies available in BBG

    Extraction & Purification of enzymes

    Determination of enzymatic activities

    Determination of activities and stabilities of biocatalysts

    Improvement of the biocatalyst: Immobilization & modification of enzymes

    Analyses of substrates and products according to european directives

    Extraction, Separation, & Purification of products mediated or not of a selective biotransformation: products not separable by physical methods such as lipids, phospholipids, antioxidants, etc.

     

    Processes catalyzed by enzymes (lipases, proteases, cellulases, phospholipases). Puesta a punto otras por encargo. Ingeniería del medio, de la reacción, del biocatalizador y del reactor para el diseño de un proceso óptimo a medida de cada materia prima (pureza, origen, inhibidores, etc).

    Optimización y Desarrollo de procesos (incluye escalado y diseños a escala piloto) de obtención de compuestos para química fina, aditivos alimentarios, emulgentes, antioxidantes, cosméticos, fármacos quirales, etc.

    Procesos desarrollados/patentados por el BBG

    Síntesis de selectiva de emulgentes de grado alimentario (derivados de monosacáridos y polioles)

    Síntesis de grasas semisólidas libres de ácidos trans

    Síntesis selectiva de lípidos estructurados (Light, ricos en PUFA tipo omega-3..)

    Síntesis selectiva de fosfolípidos estructurados (Light, ricos en PUFA tipo omega-3..)

    Producción de biodiesel a partir de aceites de 1ª y última generación. Dos procesos: en tanque agitado y sistema en continuo.

    Caracterización química, física y funcional de todas las materias primas y todos los productos

    Cromatografía de gases-masas, de líquidos-masas, RMN, IR, estabilidad oxidativa, perfiles de fusión y cristalización, plasticidad de grasas, viscosidad, contenido en agua por Karl Fisher, densidad, etc.

    Caracterización de lípidos y biodiesel conforme a normativa europea (composición en FA, glicéridos, FAMEs y FAEEs, estabilidad oxidativa y acidez libre).

    Con grupos colaboradores: Pruebas funcionales en animales y humanos (colesterol, diabetes, hipertensión, síndrome metabólico, cáncer, etc).

     

     

     

  • Lines and research projects

    Research lines

      Energy


      Selective Synthesis


      Horizontal topics


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