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RevistaElectrochemistry Communications

Direct heterogeneous electron transfer reactions of fungal laccases at bare and thiol-modified gold electrodes

Autores:Marcos Pita Martínez, Sergey Shleev , Tautgirdas Ruzgas , Víctor Manuel Fernández López, Alexander I. Yaropolov , Lo Gorton
Grupos de investigación:BioElectroCatalisis
Mediatorless (direct) electron transfer between bare and thiol-modified gold electrodes and fungal laccases from different sources has been demonstrated. The electrochemical activity of the enzymes from basidiomycetes Trametes hirsuta, Trametes ochracea, and Cerrena maxima under aerobic and anaerobic conditions can clearly be observed using cyclic voltammetry and spectroelectrochemistry. Bioelectroreduction of oxygen by T. hirsuta laccase immobilized on amino-thiophenol-modified goldelectrodes, starting at +625 mV vs. NHE, is demonstrated and differences in bioelectrocatalysis of the enzyme immobilized on bare and thiol-modified electrodes are shown. It was found that hydrogen peroxide was one of the products of oxygen electroreduction on gold electrodes modified with fungal laccases, whereas no significant peroxide formation was observed for T. hirsuta laccase immobilised on thiol-modified gold electrodes. Thus, a hypothesis about two different mechanisms of oxygenelectroreduction by fungal laccases adsorbed on bare and thiol-modified electrodes is proposed.
Palabras clave:Laccase; Redox potential; T1, T2, and T3 sites; Gold electrode; Cyclic voltammetry
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