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RevistaJournal of Colloid and Interface Science

Syntesis of cobalt ferrite core/metallic shell nanoparticles for the development of a specific PNA/DNA biosensor

Autores:Marcos Pita Martínez, Jose María Abad Pastor, Cristina Vaz Dominguez, Carlos Briones , Eva Mateo-Martí , José A. Martín-Gago , María del Puerto Morales , Víctor Manuel Fernández López
Grupos de investigación:BioElectroCatalisis
Controlled synthesis of cobalt ferrite superparamagnetic nanoparticles covered with a gold shell has been achieved by an affinity and trap strategy. Magnetic nanoparticles are functionalized with a mixture of amino and thiol groups that facilitate the electrostatic attraction and further chemisorption of goldnanoparticles, respectively. Using these nanoparticles as seeds, a complete coating shell is achieved bygold salt-iterative reduction leading to monodisperse water-soluble gold-covered magnetic nanoparticles, with an average diameter ranging from 21 to 29 nm. These constitute a versatile platform for immobilization of biomolecules via thiol chemistry, which is exemplified by the immobilization of peptide nucleic acid (PNA) oligomers that specifically hybridize with complementary DNA molecules in solution. Hybridation with DNA probes has been measured using Rhodamine 6G fluorescence marker and the detection of a single nucleotide mutation has been achieved. These results suggest the PNA-nanoparticles application as a biosensor for DNA genotyping avoiding commonly time-consuming procedures employed.
Palabras clave:Magnetic nanoparticle; Gold shell; Peptide nucleic acid; DNA–PNA hybridization; Mutation detection; Rhodamine 6G
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