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Improving the yields and reaction rate in the ethanolysis of soybean oil by using mixtures of lipase CLEAs

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Margarita Díaz Ramos1, Letícia Passos Miranda1, Roberto Fernandez-Lafuente2*, William Kopp3, Paulo Waldir Tardioli1*
1   Postgraduate Program in Chemical Engineering, Department of Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luís, km 235, 13565-905, São Carlos, SP, Brazil;
2   Departmento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC, 28049 Madrid, Spain; 
3   Kopp Technologies (KTech), Rua Alfredo Lopes, 1717, Jardim Macarengo, 13560-190 São Carlos, SP, Brazil; 

Abstract: Due to the heterogeneity of the oils, the use of mixtures of lipases with different activity for a large number of glycerol-linked carboxylic acids that composes the substrate has been proposed as a better alternative than the use of one specific lipase preparation in the enzymatic synthesis of biodiesel. In this work, mixtures of lipases from different sources were evaluated in their soluble form in the ethanolysis of soybean oil. A mixture of lipases (50% of each lipase, in activity basis) from porcine pancreas (PPL) and Thermomyces lanuginosus (TLL) gave the highest FAEE yield (around 20 wt.%) while the individual lipases gave FAEE yields 100 and 5 times lower, respectively. These lipases were immobilized individually by cross-linked enzyme aggregates (CLEAs) technique, yielding biocatalysts with 89 and 119% of expressed activity, respectively. A mixture of these CLEAs (also 50% of each lipase, in activity basis) gave 90.4 wt.% FAEE yield, while using separately CLEAs of PPL and TLL the FAEE yields were 84.7 and 75.6 wt.%, respectively, under the same reaction conditions. The mixture of CLEAs could be reused (five cycles of 6 hours) in the ethanolysis of soybean oil in a vortex flow type reactor yielding a FAEE yield higher than 80% of that of the first batch.
Palabras clave:Porcine pancreas lipase; Thermomyces lanuginosus lipase; mixture of CLEAs, soybean oil ethanolysis; vortex flow reactor; biodiesel
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