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PublicationProcess Biochemistry
Year2020
Volume
Pages
International

Parameters necessary to define an immobilized enzyme preparation.

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimization of biocatalysts and bioprocesses
Joseph Boudrant1, John M. Woodley2 and Roberto Fernandez-Lafuente3,*

1Laboratoire Réaction et Génie des Procédés, UMR CNRS 7224, University of Lorraine, ENSAIA, 54518, Vandoeuvre Cedex, France
2Chemical and Biochemical Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark
3Departamento de Biocatálisis. ICP-CSIC, Campus UAM-CSIC, Madrid, Spain


Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as well as potential recycle. Whilst much has been reported in the scientific literature about enzyme immobilization methods, in many cases the protocol leads to losses in enzyme activity. In this review we outline the reasons for loss of activity during immobilization and highlight suitable diagnostic tests to elucidate the precise cause and thereby methods to restore activity. The need for standardized reporting of immobilization methods is also emphasized as a means of benchmarking alternative approaches. 
 

Keywords:Enzyme stabilization, Enzyme immobilization, Enzyme inactivation, facts and artifacts, yield determination
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