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RevistaBiocatalysis and Agricultural Biotechnology
Año2019
Volumen22
Páginas101411
Internacional

Recovery of starch from cassava bagasse for cyclodextrin production by sequential treatment with alpha-amylase and cyclodextrin glycosyltransferase

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Mayerlenis Jimenez Rojas1, Murilo Amaral-Fonseca1, Roberto Fernandez Lafuente2*, Raquel de Lima Camargo Giordano1, Paulo Waldir Tardioli1*
 1Programa de Pós-Graduação em Engenharia Química, Departamento de  Engenharia Química, Universidade Federal de São Carlos, Rodovia Washington Luís, 13565-905 São Carlos, SP, Brazil; 
 2Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC Madrid, Madrid 28049, Spain; 
 
This work aimed at the enzymatic recovery of starch from cassava bagasse using alpha-amylase and the production of cyclodextrins (CDs) by cyclodextrin glycosyltransferase (CGTase). Cassava bagasse containing 50% (w/w) starch (dry basis) was hydrolyzed by alpha-amylase until a maximum starch recovery as a maltooligosaccharide solution. Afterward, this solution was used for the production of CDs using CGTase as biocatalyst. Optimal conditions for starch extraction using alpha-amylase were 60 °C and 0.15 mL enzyme/kg dry bagasse, with 93% starch recovery. Several operational parameters were evaluated to optimize the CD yields, such as starch dextrose equivalent (DE), temperature and CGTase loading. The starch DE did not significantly influence the production of CDs, resulting in a similar yield for all experiments (43%). The highest productivity of CDs (93.6 mg of CDs per enzyme Unit) was obtained using an enzyme loading of 2.5 U/g starch for 1 h at 60°C and pH 6.0. After 6 h of reaction at 60oC and pH 6.0, the CD yield reached a value of 50%. These findings did indicate that cassava bagasse is a promising feedstock for the production of CDs using this bi- enzymatic system.
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