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RevistaInternational Journal of Biological Macromolecules
Año2019
Volumen141
Páginas313-324
Internacional

Further stabilization of lipase from Pseudomonas fluorescens immobilized on octyl coated nanoparticles via chemical modification with bifunctional agents

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
 Nathalia Saraiva Rios1,2; Eva Gomes Morais3; Wesley dos Santos Galvão4; Davino M. Andrade Neto4,7; José Cleiton Sousa dos Santos5; Felipe Bohn6; Marcio A. Correa7; Pierre Basílio Almeida Fechine4; Roberto Fernandez-Lafuente2,* , Luciana Rocha Barros Gonçalves1,*
1 Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, 60455-760 Fortaleza, CE, Brazil.
2 Departamento de Biocatálisis. ICP-CSIC, Campus UAM-CSIC, 28049 Madrid, Spain.
3 Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, Bloco 907, 60455-760 Fortaleza, CE, Brazil.
4Departamento de Química Analítica e Físico-Química, Centro de Ciências, Universidade Federal do Ceará, Av. Mister Hull s/n, Pici, 60455-760, Fortaleza, CE, CP 12200, Brazil
 5 Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Campus das Auroras, Bloco A, Rua José Franco de Oliveira, s/n, 62790790 Redenção, CE, Brazil.
6 Departamento de Física, Universidade Federal do Rio Grande do Norte, 59078-900 Natal, RN, Brazil.
7 Instituto Federal de Educação, Ciência e Tecnologia do Ceará – Campus Camocim – Rua Raimundo Cals, 2041, 62400-000, Camocim – CE, Brazil.



The lipase from Pseudomonas fluorescens (PFL) was adsorbed on superparamagnetic NiZnFe2O4 octyl-nanoparticles via interfacial activation, producing the biocatalyst OCTYL-NANO-PFL. In order to further improve the stability of the immobilized lipase, the immobilized enzyme biocatalyst was chemically modified with different concentrations of diverse bifunctional molecules (glutaraldehyde (GA), divinylsulfone (DVS) or p-benzoquinone (BQ)). The concentrations of bifunctional agents were varied (0.5, 1, 2.5 and 5 % (v/v for GA and DVS and w/v for BQ)). The results showed a greatly improved stability after chemical modification with all bifunctional molecules, mainly with 5 % (v/v) GA or 1 % (v/v) DVS. The biocatalysts OCTYL-NANO-PFL-GA 5 % and -DVS 1 % were about 60 folds more stable at pH 7 than the unmodified preparation and, at pH 5, more than 200 folds for 5 % GA modified enzyme. The most stable BQ treated biocatalysts,  OCTYL-NANO-PFL-BQ 0.5 %, was about 8.3 more stable than OCTYL-NANO-PFL at pH 7, while was 20 fold more stable at pH 9.

Palabras clave:interfacial activation, intermolecular crosslinking, solid phase chemical modification, prevention of enzyme release
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