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RevistaInternational Journal of Molecular Sciences
Año2019
Volumen20
Páginas4018
Internacional

Immobilization of Lipase A from Candida antarctica onto Chitosan-coated Magnetic Nanoparticles

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Rodolpho R. C. Monteiro 1, Paula J. M. Lima 1, Bruna B. Pinheiro 1, Tiago M. Freire 32, Lillian M. U. Dutra 32, Pierre B. A. Fechine 32, Luciana R. B. Gonçalves 1, Maria C. M. de Souza 43, José C. S. dos Santos 34,* and Roberto Fernandez-Lafuente 4,*
1   Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, CEP 60455760, Fortaleza, CE, Brazil; montteirorrc@gmail.com (R.R.C.M.); paula.morais.lima@gmail.com (P.J.M.L.); bruna.b.pinheiro@hotmail.com (B.B.P.); lrg@ufc.br (L.R.B.G.)
23  Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará, Campus do Pici, Bloco 940, CEP 60455760, Fortaleza, CE, Brazil; tiagomfreire.ufc@gmail.com (T.M.F.); lmudutra@hotmail.com (L.M.U.D.);  fechine@ufc.br (P.B.A.F.)
34  Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Campus da Auroras, CEP 62790970, Redenção, CE, Brazil; mariacristiane@unilab.edu.br (M.C.M.S.); jcs@unilab.edu.br (J.C.S.S.)
45  Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC Madrid, Spain; rfl@icp.csic.es (R.F-L.)
 


Abstract: In this communication, lipase A from Candida antarctica (CALA) was immobilized by covalent bonding on magnetic nanoparticles coated with chitosan and activated with glutaraldehyde, labelled CALA-MNP, (immobilization parameters: 84.1% ± 1.0 for immobilization yield and 208.0 ± 3.0 U/g ± 1.1 for derivative activity). CALA-MNP biocatalyst was characterized by X-Ray Powder Diffraction (XRPD), Fourier Transform Infrared (FTIR) spectroscopy, Thermogravimetry (TG) and Scanning Electron Microscope (SEM), proving the incorporation of magnetite and the immobilization of CALA in the chitosan matrix. Besides, the immobilized biocatalyst showed a half-life 8-11 times higher than that of the soluble enzyme at pH 5-9. CALA showed the highest activity at pH 7, while CALA-MNP presented the highest activity at pH 10. The immobilized enzyme was more active than the free enzyme at all studied pH values, except pH 7.
Palabras clave:lipase A from Candida antarctica; chitosan; magnetic nanoparticles; characterization
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