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RevistaCatalysts
Año2019
Volumen9
Páginas622
Internacional

Dextran aldehyde in biocatalysis: more than a mere immobilization system

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Veymar G. Tacias-Pascacio 1,2, Claudia Ortiz 3, Nazzoly Rueda 3, Ángel Berenguer-Murcia 4, Niuris Acosta 5, Inmaculada Aranaz 5, Concepción Civera 5, Roberto Fernandez-Lafuente 6,* and Andrés R. Alcántara 5*  
1   Tecnológico Nacional de México/ Instituto Tecnológico de Tuxtla Gutiérrez, Carretera Panamericana Km. 1080, Tuxtla Gutiérrez, Chiapas, Mexico. vey_pascacio@live.com
2     Facultad de Ciencias de la Nutrición y Alimentos. Universidad de Ciencias y Artes de Chiapas, Chiapas. México;
3   Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia; ortizc@uis.edu.co (C. O); nazzoly@gmail.com (N. R.)
4   Departamento de Química Inorgánica e Instituto Universitario de Materiales, Universidad de Alicante, Alicante, Spain; a.berenguer@ua.es
5   Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain; facosta@ucm.es (N. A.); iaranaz@ucm.es (I. A.); mccivera@ucm.es (C. C.); andalcan@ucm.es (A. R. A.)
6   Departamento de Biocatálisis. ICP-CSIC. Campus UAM-CSIC. Madrid. Spain; rfl@icp.csic.es.


 Dextran aldehyde (dexOx), resulting from the periodate oxidative cleavage of 1,2-diol  moiety inside dextran, is a polymer very useful in many areas, as macromolecular carrier for drug  delivery and other biomedical applications. Particularly, it has been widely used for chemical  engineering of enzymes, with the aim of designing better biocatalysts, possessing improved  catalytic properties, making them more stable and/or active for different catalytic reactions. This  polymer possesses a very flexible hydrophilic structure, which, after chemical reduction, becomes inert; therefore, dexOx comes to be highly versatile in biocatalysts design. This paper presents an overview of the multiple applications of dexOx in applied biocatalysis, e.g., to modulate the adsorption of biomolecules on carrier surfaces in affinity chromatography and biosensors design,  to serve as a spacer arm between a ligand and the support in biomacromolecule immobilization procedures or to generate artificial microenvironments around the enzyme molecules or to stabilize  multimeric enzymes by intersubunit crosslinking, among many other. 
Palabras clave:dextran; dextran-aldehyde; enzyme immobilization; enzyme modification
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