Puede utilizar el filtro de búsqueda del panel izquierdo para acotar los resultados
Filtro
Tipo de publicación
Todos Libros Revistas
Título
Autor
Palabras clave
ISBN
Acceso DOI
Acceso digital CSIC
Buscar
Datos técnicos
RevistaINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Año2019
Volumen134
Páginas936-945
Internacional

Comparison of the immobilization of lipase from Pseudomonas fluorescens on divinylsulfone or p-benzoquinone activated support

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Nathalia Saraiva Rios¹,2; Davino M. Andrade Neto3; José Cleiton Sousa dos Santos4; Pierre Basílio Almeida Fechine3; Roberto Fernández-Lafuente2*; Luciana Rocha Barros Gonçalves1*
 
1 Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, 60455-760 Fortaleza, CE, Brazil.
2Departamento de Biocatálisis. ICP-CSIC, Campus UAM-CSIC, Madrid, Spain.
3Departamento de Química Analítica e Físico-Química, Centro de Ciências, Universidade Federal do Ceará, Av. Mister Hull s/n, Pici, 60455-760, Fortaleza, CE, CP 12200, Brazil
4Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, 62785000 Acarape, CE, Brazil.



NiZnFe2O4 superparamagnetic nanoparticles were coated with silica by impregnation with tetraethoxysilane (TEOS) and further activated with divinylsulfone (DVS) and p-benzoquinone (BQ) for covalent immobilization lipase from Pseudomonas fluorescens (PFL), producing the biocatalysts TEOS-NANO-DVS-PFL and TEOS-NANO-BQ-PFL. The optimal conditions for enzyme immobilization were found to be pH 7 and 0.1 M of both activating reagents. PFL was also immobilized on TEOS nanoparticles without any activation as a reference (TEOS-NANO-PFL). Results indicated that TEOS could be released from the nanoparticles at alkaline pH value. Optimal TEOS-NANO-PFL exhibited a recovered activity of 55 % and a t1/2(60ºC) of just over 150 min; while TEOS-NANO-DVS-PFL showed 82 % of activity recovered and t1/2(60ºC) of 225min; being the TEOS-NANO-BQ-PFL the biocatalyst offering the best results (89 % of recovered activity and a half life over 1440 min), the maximum enzyme load was » 300 U/g.

Palabras clave:ADSORPTION, Enzyme stabilization, lipase, Enzyme immobilization, covalent binding
logo de CSIC