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RevistaFood Chemistry

Rapid and high yield production of phospholipids enriched in CLA via acidolysis: the critical role of the enzyme immobilization protocol

Autores:Carlos Manuel Verdasco Martín, Roberto Fernandez-Lafuente, Cristina Otero Hernández
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos, Biocatalisis y Bioenergía (GBB)
Carlos M. Verdasco-Martín; Carlos Corchado Lopo;
Roberto Fernández-Lafuente; Cristina Otero

Abstract: Phospholipids (PL) rich in conjugated linolenic acid (CLA) have
important health effects. Yields of phosphatidylcholine (PC) acidolysis
with CLA use to be limited to <30%, due to competitive side-hydrolysis.
Duolite A658- Lecitase is a very suitable biocatalyst for this reaction.
In this study, PC hydrolysis has been practically eliminated using
extremely dried lyophilized PC (279 ± 4 mg water/Kg PC), obtaining close
to 100% molar yield of modified PC (72.3% CLA) with Duolite-Lecitase in
24h, the highest yield reported in the literature for this reaction. It
has been better improved by changing the immobilization support, using
three food grade hydrophobic supports (Styrene, and two Octadecyl
methacrylates (OM and OMC)). In only 2h, with a 1/12 PC/CLA molar ratio
at 50ºC, similar almost quantitative yields of PC with 74.4% CLA content
has been obtained using OM-Lecitase. The fatty acid composition of
modified PCs is not affected by the enzyme immobilization protocol
Palabras clave:phosphatidylcholine; enzyme modulation via immobilization; Lecitase® 41 42 Ultra; CLA; structured phospholipids; nutraceutical
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