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RevistaMolecular Catalysis
Año2019
Volumen473
Páginas110405
Internacional

LECITASE ULTRA: A PHOSPHOLIPASE WITH GREAT POTENTIAL IN BIOCATALYSIS

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Jose J. Virgen-Ortíz1, José C.S. dos Santos2, Claudia Ortiz3, Ángel Berenguer-Murcia4, Oveimar Barbosa5, Rafael C. Rodrigues6, Roberto Fernandez-Lafuente 7,*
 
1CONACYT - Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) - Consorcio CIDAM, Km. 8 Antigua Carretera a Pátzcuaro s/n, 58341 Morelia, Michoacán, México.
2Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, CEP 62790-970, Redenção, CE, Brazil.
3Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia
4Instituto Universitario de Materiales, Departamento de Química Inorgánica, Universidad de Alicante, Campus de San Vicente del Raspeig, Ap. 99 - 03080 Alicante, Spain.
5Departamento de Química, Facultad de Ciencias. Universidad del Tolima, Ibagué, Colombia.
6Biocatalysis and Enzyme Technology Lab, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, Porto Alegre, RS, Brazil.
7 Departamento de Biocatálisis ICP-CSIC. Campus UAM-CSIC. Cantoblanco. 28049 Madrid. Spain.



Lecitase Ultra is a chimera produced by the fusion of the genes of the lipase from Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum. The enzyme was first designed for the enzymatic degumming of oils, as that problem was not fully resolved before. It is commercialized only as an enzyme solution by Novo Nordisk A/S. This review shows the main uses of this promising enzyme. Starting from the original degumming use, the enzyme has found applications in many other food modification applications, like production of structured phospholipids (e.g., derivatives of phosphatidylcholine), tuning the properties of flour, etc. Moreover, the enzyme has been used in fine chemistry (resolution of racemic mixtures), in the production of aromas and fragrances, polymers modification, etc. Some papers show the use of the enzyme in biodiesel production. Moreover, we present the different technologies applied to obtain a suitable immobilized biocatalyst, remarking the immobilization via interfacial activation and how heterofunctional acyl supports may solve some of the limitations. Immobilized enzyme physical and chemical modifications have also been presented. Finally, Lecitase Ultra has been one of the model enzymes in a new strategy to coimmobilize lipases and other less stable enzymes.

Palabras clave:Enzyme stabilization, Enzyme immobilization, interfacial activation, enzyme modulation, phospholipase, oil degumming, structured lipids
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