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RevistaBiochemial Engineering Journal

Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Sabrini N. S. Ávila, Melissa L. E. Gutarra, Roberto Fernandez-Lafuente, Elisa D. C.
Cavalcanti and Denise M. G. Freire
Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. A, Sl. 549, Ilha do Fundão, 21949-900, Rio de Janeiro, Brazil
Federal University of Rio de Janeiro, Campus Xerém, Estrada de xerém, 27 CEP 25245-390, Duque
de Caxias, Rio de Janeiro, Brazil
Departamento de Biocatálisis. ICP-CSIC, Campus UAM-CSIC Madrid, Spain
Federal University of the State of Rio de Janeiro, Postgraduate Program in Food and Nutrition, Rio
de Janeiro, Brazil.
A multipurpose fixed-bed bioreactor was evaluated, to simplify the process of producing lipase and its application in biocatalysis. Rhizomucor miehei lipase was produced by solid-state fermentation (SSF) in the fixed-bed bioreactor and generated a fermented solid with 30 U/g lipase activity and a synthesis capacity of 80 % (conversion at 6 h of reaction in a standard stirred reactor). The fermented solid was then dried by forced aeration in the same column (1.0 L air/min, 35 °C) and the dried fermented solid (5 wt.% water) showed the same synthesis capacity as the freeze-dried standard, with high storage stability at 4 °C and 25 °C (14 months with no decrease in synthesis capacity). The reaction performed in the multipurpose bioreactor (one-pot system) showed relatively low conversion (30 % of the conversion obtained in the stirred reactor at 6 h) when only babassu cake was employed as the fermentation substrate, due to the preferred pathways formed as a consequence of bed retraction during drying. Therefore, SSF was carried out with a mixture of 40:60 wt.% babassu cake and palm fiber (used to give structure to the fermented solid and prevent retraction during drying), and the reaction in the one-pot system reached a maximum conversion of 71 %.

Palabras clave:lipase, Biocatalysis, Solid-State Fermentation, Rhizomucor miehei, Fixed-bed bioreactor, one-pot system.
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