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RevistaJ. of the Amer. Oil Chem. Soc
Año2013
Volumen90
Páginas1859–1863
Internacional

ENZYMATIC INTERESTERIFICATION BETWEEN PINE SEED OIL AND A HYDROGENATED FAT TO PREPARE SEMI-SOLID FATS RICH IN PINOLENIC ACID AND OTHER POLYUNSATURATED FATTY ACIDS

Autores:Cristina Otero Hernández
Grupos de investigación:Biocatalisis y Bioenergía (GBB)
Enzymatic Interesterification Between Pine Seed Oil and a Hydrogenated Fat to Prepare Semi-Solid Fats Rich in Pinolenic Acid and Other Polyunsaturated Fatty Acids Cristina Otero • Pablo Ma´rquez • Manuel Criado • Estela Herna´ndez-Martı´n
J Am Oil Chem Soc (2013) 90:81–90
 
Abstract Enzyme catalyzed interesterification (EIE) of pine seed oil (PSO) and a fully hydrogenated soybean oil (FHSBO) were studied in batch reactors in solvent-free media to prepare different semisolid fats rich in polyunsaturated fatty acids (PUFA). Optimal operation conditions found were: 10 % (w/w) enzyme loading, 75 C and magnetic agitation at 300 rpm. Quasi-equilibrium conditions were reached after 2, 3 and 6 h, when immobilized lipases from Thermomyces lanuginosus (Lipozyme TL IM), Candida antarctica B. (Novozym 435) and Rhizomucor miehei (Lipozyme RM IM) from Novozymes A/S (Bagsvaerd, Denmark) were employed, respectively. Similar distributions of unsaturated to saturated fatty acid (UFA/SFA) residues along the glycerol backbone of the fat products were obtained with both non-selective and sn-1(3) regioselective lipases due to significant spontaneous acyl migration during the reaction. The products had higher UFA/SFA ratios at the sn-2 position (2.4–2.5, 1.4–1.7, and 0.5–0.8 for the trials involving 20, 40 and 70 % FHSBO, w/w, respectively) than the corresponding physical blends (0.8, 0.4 and 0.5, respectively). Fat products containing 3.1–11.6 % (w/w) pinolenic acid (Pn) and 16.1–35.7 % (w/w) linoleic acid (L) at the sn-2 position were prepared. The free acid contents of EIE products prepared with Lipozyme TL IM and Novozym 435 were 6.1–6.4 and 2.5–2.6, respectively. Residual activities of Lipozyme TL IM and Novozym 435 diminish by ca. 20 % after 9 reaction cycles.
Palabras clave:phosphatidylcholine; hydrolysis; lipases; phospholipase; lysophosphatidylcholine
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