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RevistaJ Chem Technol. & Biotechnol
Año2013
Volumen88
Páginas1859–1863
Internacional

ENZYME - CATALYSED HYDROLYSIS OF PHOSPHATIDYLCHOLINE FOR THE PRODUCTION OF LYSOPHOSPHATIDYLCHOLINE

Autores:Cristina Otero Hernández
Grupos de investigación:Biocatalisis y Bioenergía (GBB)
Ramiro Baeza-Jim´enez,a Leticia X. L ´ opez-Mart´ınez,b Cristina Otero,a∗
In-Hwan Kimc and Hugo S. Garc´ıad∗
J Chem Technol Biotechnol 2013; 88: 1859–1863
Abstract:
BACKGROUND Production of lysophosphatidylcholine (LPC) via enzyme-catalysed hydrolysis was studied. Three enzymeswere
employed to conduct the reactions at different temperatures. The starting material, phosphatidylcholine (PC), was dispersed in
water (system A) and in ethanol (system B) to define the reaction mixture to carry out the trials.
RESULTS: It was found that themedia employed and the type of biocatalyst (free or immobilized), clearly determine the kinetics
of the reactions. PC was well dissolved in ethanol but an opaque emulsion was obtained when it was dissolved in water.
Immobilized PLA1 and Novozym 435 were able to convert PC into LPC in ethanol, with yields of 50% and 58.51%, respectively,
after 48 h at 50 ◦C. The highest degree of hydrolysis (70%) was reached with Lipase PS after 48 h at 60 ◦C in water.
CONCLUSIONS: Both reaction media enabled fairly good yields but water was better. The present work proposes a simple
reaction scheme compared with other reports in the literature where different substrates, additives and polar solvents have
been employed. LPC has interesting properties as a bioemulsifier, which led us to develop new methods for its production.
Palabras clave:hosphatidylcholine; hydrolysis; lipases; phospholipase; lysophosphatidylcholine
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