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RevistaEur Food Res Technol
Año2013
Volumen236
Páginas435-440
Internacional

OPTIMIZATION OF STRUCTURED DIACYLGLYCEROLS PRODUCTION CONTAINING -3 VIA ENZYME-CATALYZED GLYCEROLYSIS OF FISH OIL

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Grupos de investigación:Biocatalisis y Bioenergía (GBB)
Optimization of structured diacylglycerols production containing x-3 fatty acids via enzyme-catalysed glycerolysis of fish oil Kelhy Miranda • Ramiro Baeza-Jime´nez • Juan A. Noriega-Rodrı´guez • Hugo S. Garcı´a • Cristina Otero*
 
Abstract The main objective of the present work was the production of structured diacylglycerols (DAG) by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Different enzyme loads (5, 7, 10, 13 and 15 %, with respect to the total weight of substrates), temperatures (40, 44, 50, 56 and 60 C) and molar ratios of substrates (1, 1.4, 2, 2.6 and 3, oil/glycerol) were explored by the response surface methodology in order to optimize the reaction system. Temperature showed the highest significant effect (P\0.05) on the glycerolysis reaction. Good agreement on the experimental data and the second-order polynomial regression was obtained (R2 = 0.85). Optimal conditions for the production of DAG were established by the model using 15 % enzyme load at 60 C for a 2:1 (oil/glycerol) substrates molar ratio, reaching a 50 % yield, with 89.37 % n-3 PUFA content, namely, EPA (11.32 %), DPA (8.34 %) and DHA (69.71 %).
Palabras clave:Omega-3, DHA, essential acids, lipids, partial glycerides, EPA, fish oil, glycerolisis
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