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RevistaThe FASEB Journal

Effects of DHA on orchidectomy-associated alterations in vascular responses

Autores:Cristina Otero Hernández
Grupos de investigación:Biocatalisis y Bioenergía (GBB)
Effects of DHA on Orchidectomy-
Associated Alterations in Vascular
Diva M. Villalpando1,2, Lara del Campo1,2, Rocío Navarro1, Carlota Largó2,
David Muñoz1, María Tabernero2, Cristina Otero3, Hugo S. García4 and
Mercedes Ferrer1,2
Author Affiliations
The loss of gonadal function induces vascular dysfunction by altering the release
and action of NO, prostanoids and reactive oxygen species. Although, the
cardioprotective effect of n-3 polyunsaturated fatty acids has been documented,
the specific effect of docosahexaenoic acid (DHA) in vascular function of
orchidectomized rats has been scantly investigated. Therefore, the objective of
this study was to analyze whether a DHA-supplemented diet prevents vasodilator
and vasoconstrictor alterations induced by impaired gonadal function. For this
purpose, mesenteric arteries from control (C) and orchidectomized (CX) 6 monthsold
Sprague-Dawley rats were used. Control rats were fed with a control diet (V-C),
while CX rats were fed with a control diet (V-CX) or one containing 2% (w/w) DHA
(DHA-CX). Hydrogen peroxide and antioxidant activity were analyzed in serum
samples. Mesenteric artery segments were used to analyze: (i) the levels of NO and
thromboxane A2 (TXA2); (ii) the vasodilator response induced by acetylcholine
(Ach) in the absence and presence of L-NAME and indomethacin, the respective
inhibitors of NO synthase and cyclooxygenases; (iii) the vasodilator response to
the NO donor, sodium nitroprusside (SNP); and (iv) the effect of L-NAME on the
contractile response induced by noradrenaline (orchidectomy increased the production of hydrogen peroxide while decreased the antioxidant activity and the DHA-diet prevented these alterations. The release of NO was decreased in arteries from CX rats and it was restored by the DHA-diet.
The Ach-induced response was decreased by L-NAME plus indometacin in arteries
from all groups, being the smallest decrease that of arteries from the V-CX group.
In KCl-precontracted arteries, the SNP-induced relaxation was greater in arteries
from V-CX group than those of C or CX-DHA groups. Regarding NA-induced
response, L-NAME failed to modify that response in V-CX group, while in arteries
from C and CX-DHA L-NAME increased the NA-induced response. These results
show that orchidectomy promotes oxidative stress that accounts for a decreased
NO and an increased TXA2 release. The DHA-supplemented diet in CX rats
restored the redox status by decreasing hydrogen peroxide production and by
increasing the antioxidant activity; in turn, the production of NO was recovered.
Dietary DHA supplementation in CX rats increased the participation of NO in the
Ach-induced vasodilation and in the NA-induced vasoconstriction.
Palabras clave:Omega-3, cardiovascular function, DHA, essential acids
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