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PublicationMacromolecular Reaction Engineering

Effects of Reaction Operation Policies on Properties of Core-Shell Polymer Supports used for Preparation of Highly Active Biocatalysts

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimization of biocatalysts and bioprocesses
Martina Costa Cerqueira Pinto, Nathany Lisbôa de Souza e Castro, Eliane Pereira
Cipolatti, Roberto Fernandez-Lafuente, Evelin Andrade Manoel, Denise Maria
Guimarães Freire, José Carlos Pintoa
aPrograma de Engenharia Química, COPPE, Universidade Federal do Rio de Janeiro,
Rio de Janeiro, Brazil.
bDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de
Janeiro, Rio de Janeiro, Brazil.
cDepartment of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, 28049,
Madrid, Spain.
dDepartamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade
Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
New polymer particles produced through combined suspension/emulsion
polymerizations and exhibiting distinct morphologies were developed for the synthesis
of high performance enzymatic biocatalysts. Particularly, different operation strategies
were adopted during the polymerization step to produce particles with high specific area
and porosity. Polymer particles were employed as supports for the immobilization of
lipase B from Candida antarctica and some immobilization parameters, such as yield of
immobilization and retention of activity, were evaluated. The performances of the
obtained biocatalysts were assessed in hydrolysis and esterification reactions. It was
observed that the manipulation of the suspension polymerization stage allowed the
production of particles with very high specific area and porosity. Additionally, it
became evident that the use of core-shell particles as supports allowed the production of
more active biocatalysts, indicating that the core-shell structure generates a favorable
microenvironment that enhances the final performance of the biocatalysts. Finally, the
use of the support with the highest specific area allowed the production of highly active
biocatalysts for both hydrolysis (Ahyd = 34.7 ± 3.8 U/g) and esterification (Aest =
3564.6 ± 581 U/g) reactions, when compared to the commercial biocatalyst Novozym
435 (Ahyd = 7.6 ± 1.8 U/g; Aest = 2384.7 ± 307.2 U/g).

Keywords:Combined suspension and emulsion polymerization; core-shell particles; immobilization; lipase B from Candida antarctica; biocatalyst
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