Puede utilizar el filtro de búsqueda del panel izquierdo para acotar los resultados
Filtro
Tipo de publicación
Todos Libros Revistas
Título
Autor
Palabras clave
ISBN
Acceso DOI
Acceso digital CSIC
Buscar
Datos técnicos
RevistaPROCESS BIOCHEMISTRY
Año1970
Volumen
Páginas
Internacional

Production of lactulose from whey using beta-galactosidase from Kluyveromyces lactis immobilized in glutaraldehyde-chitosan

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Tiago Lima de Albuquerquea, Sandy Danielle Lucindo Gomesa, Alan Portal D’almeidaa, Roberto Fernandez-Lafuenteb, Luciana Rocha Barros Gonçalvesa, Maria Valderez Ponte Rochaa,*.
aUniversidade Federal do Ceará, Departamento de Engenharia Química, Campus do Pici, Bloco 709, 60455-760, Fortaleza, CE, Brazil.
b Departamento de Biocatálisis. ICP-CSIC, Campus UAM-CSIC Madrid, Spain
 
 
The aim of this work was to establish optimal conditions for the enzymatic production of lactulose by β-galactosidase from Kluyveromyces lactis using cheese whey as feedstock. Initially, the production of this disaccharide was evaluated using free β-galactosidase and synthetic medium (containing lactose and fructose), and the optimal conditions for the production of lactulose were as follows: lactose/fructose ratio of 15/30% (w/v); total initial concentration of lactose and fructose of 200 g/L; and operational conditions at 50 °C, pH 7. With the addition of 0.01% w/v Mg2+, the system reached a maximum lactulose production of 22.6 g/L in 2 h of reaction time with a productivity of 11.3 g/(L.h). Then, β-galactosidase was immobilized on chitosan activated with glutaraldehyde to obtain highly active and stable catalysts for lactulose synthesis using cheese whey and fructose as the substrate. The highest production of lactulose (17.3 g/L) was achieved with the treated cheese whey for protein precipitation and was observed at a high operational stability. It was therefore concluded that it is possible to use lactose from cheese whey for the synthesis of lactulose, which could provide an appropriate use for this waste product, reducing environmental damage and cost.
 
Palabras clave:Waste reuse. Dairy industry. Enzymatic synthesis. Immobilization. Prebiotic.
logo de CSIC