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RevistaRenewable Energy

Production of lipases in cottonseed meal and application of the fermented solid as biocatalyst in esterification and transesterification reactions

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Erika C. G. Aguieiras1+*, Daniele S. N. de Barros 1+, Roberto Fernandez-Lafuente,
Denise M. G. Freire1* 5
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de
 Janeiro, Av. Athos da Silveira Ramos, 149, Zip Code 21949-909, Rio de Janeiro, RJ,
 Departamento de Biocatalisis. ICP-CSIC, C/ Marie Curie 2, Campus UAM10
CSICMadrid, Spain
Cottonseed meal is a co-product from the cotton industry that
is produced in large quantities worldwide. The aim of this work was to
add value to this by-product through the production of Rhizomucor miehei
lipases and application of the dry fermented solid in esterification
reactions between oleic acid and ethanol or methanol and
transesterification of a vegetable acid oil. Reactions were carried out
in a solvent-free system and conversions higher than 85% were obtained in
4h in the esterification reactions. The reusability of the biocatalyst
was evaluated and the washing of the biocatalyst with solvent (ethanol)
was necessary to recover the activity of the lipase, being obtained
conversion of 74% in relation to the initial conversion after 4 sucessive
reactions. The dried fermented solid was also able to catalyze the
production of ethyl and methyl esters by simultaneous
transesterification/esterification reactions of macaúba acid oil, with
production of an ester content of about 76% after 24h. This is the first
time that fermented solids produced in cottonseed meal without any
supplementation are used as biocatalyst of esterification and
transesterification reactions.

Palabras clave:fatty acid ethyl esters; cottonseed meal; dry fermented solid; lipases
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