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RevistaBiochemial Engineering Journal
Año2018
Volumen138
Páginas1-11
Internacional

KINETIC CHARACTERIZATION OF CARBONIC ANHYDRASE IMMOBILIZED ON MAGNETIC NANOPARTICLES AS BIOCATALYST FOR CO2 CAPTURE

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
S. Peirce1, M.E. Russo2*, R. Perfetto3, C. Capasso3, M. Rossi3, R. Fernandez-Lafuente4 , P. Salatino1, A. Marzocchella1
1 Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale - Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli (Italy)
2 Istituto di Ricerche sulla Combustione– Consiglio Nazionale delle Ricerche, P.le V. Tecchio 80, 80125 Napoli (Italy)
3 Istituto di Bioscienze e Biorisorse – Consiglio Nazionale delle Ricerche, Via P. Castellino 111, 80131 Napoli (Italy)
4 Instituto de Catálisis y Petrolquímica - Consejo Superior de Investigaciones Cíentificas,  Cantoblanco, 28049 Madrid (Spain)
 
 
Abstract: Carbon dioxide absorption into carbonate solutions promoted by
the enzyme carbonic anhydrase (CA, E.C. 4.2.1.1) has been proposed as
potential technology for CO2 capture. The use of solid CA-based
biocatalysts allows the enzyme recovery and reuse under continuous
operating conditions typical of industrial applications. The present
study regards the development and the kinetic characterization of a solid
CA-based biocatalyst to be used in CO2 capture processes based on
reactive absorption. A technical grade thermostable CA was immobilized by
covalent bonding on aminated paramagnetic Fe3O4 nanoparticles via
carbodiimide activation of the enzyme. The effective covalent binding of
the enzyme on the support was verified in the alkaline carbonate solution
used as solvent for CO2 absorption tests. The kinetics of the biocatalyst
was assessed by means of CO2 absorption tests in a stirred cell reactor.
The liquid solvents used in the CO2 absorption tests were 0.5 M
Na2CO3/NaHCO3 buffer (pH 9.6) at 298 and 313 K and 10% wt/wt K2CO3
solutions at different carbonate conversion degrees (0-40%). The
Danckwerts' theory for gas-liquid reactive absorption was applied to
assess the intrinsic second order kinetic constant kcat/KM for the CO2
hydration reaction catalyzed by the immobilized CA. Results pointed out
that kcat/KM ranged between 10^2 and 10^3 m^3∙kg^-1∙s^-1 and that it
increased with the temperature and with the decrease of carbonate
conversion degree.
Palabras clave:CO2 capture, carbonic anhydrase, immobilized enzyme, reactive absorption
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