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RevistaBiotechnology progress
Año2018
Volumen34
Páginas952-959
Internacional

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug-flow packed-bed continuous reacto

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Jakeline Kathiele Poppea#, Carla Roberta Mattea#, Vitoria Olave de Freitasa , Roberto
Fernandez-Lafuenteb, Rafael C. Rodriguesa, Marco Antonio Zachia Ayuba* .
a Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology
 Institute, Federal University of Rio Grande do Sul, Av. Bento Goncalves 9500, PO Box
15090, ZC 91501-970, Porto Alegre, RS, Brazil;
b Department of Biocatalysis, ICP CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049, Madrid, Spain.
 
This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The influence of the addition of glass beads to the reactor bed, evaluation of the use of different solvents, and flow rate on reaction conditions were  studied. All experiments were conducted using the best combination of lipases according to the fatty acid composition of the waste oil (combi-lipase composition: 40% of TLL, 35 % of CALB, and 25 % of RML), and soybean oil (combi-lipase composition: 22.5 % of TLL, 50 % of CALB, and 27.5 % of RML). The best general reaction conditions were found to be using tert-butanol as solvent, and the flow rate of 0.08 mL min−113 . The combi-lipase reactors operating at steady state for over 30 days  (720 h), kept conversion yields of approximately 50 %, with average productivity of 1.94 gethyl esters gsubstrate -1 h-115 , regardless of the type of oil in use.
Palabras clave:Packed-bed reactor; biodiesel; waste oil; soybean oil; combi-lipase.
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