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Immobilisation of redox enzymes on nanoporous gold electrodes: applications in biofuel cells

Autores:Urszula Salaj-Kosla , Peter O Conghaile , Marcos Pita Martínez, Roland Ludwig , Edmond Magner
Grupos de investigación:BioElectroCatalisis
Nanoporous gold (NPG) electrodes were prepared by dealloying
sputtered gold:silver alloys. Electrodes of different thicknesses
and pore sizes areas were prepared by varying the temperature
and duration of the dealloying procedure; these were
then used as supports for FAD-dependent glucose dehydrogenase
(GDH) (Glomorella cingulata) and bilirubin oxidase
(BOx) (Myrothecium verrucaria). Glucose dehydrogenase was
immobilized by drop-casting a solution of the enzyme with an
osmium redox polymer together with a crosslinked polymer,
whereas bilirubin oxidase was attached covalently through carbodiimide
coupling to a diazonium-modified NPG electrode.
The stability of the bilirubin-oxidase-modified NPG electrode
was significantly improved in comparison with that of a planar
gold electrode. Enzyme fuel cells were also prepared; the optimal
response was obtained with a BOx-modified NPG cathode
(500 nm thickness) and a GDH-modified anode (300 nm),
which generated power densities of 17.5 and 7.0 mWcm@2 in
phosphate-buffered saline and artificial serum, respectively.
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