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PublicationRenewable Energy

Influence of the raw material on the final properties of biodiesel produced using lipase from Rhizomucor miehei grown on babassu cake as biocatalyst of esterification reactions

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimization of biocatalysts and bioprocesses
Erika C. G. Aguieiras1*, Daniele S. N. de Barros1, Homero Sousa2, Roberto Fernandez-Lafuente3, Denise M. G. Freire1*
1Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Zip Code 21949-909, Rio de Janeiro, RJ, Brazil
2Companhia Refinadora da Amazônia (Grupo Agropalma), Rodovia Arthur
Bernardes 5.555, Zip Code 66825 – 000, Tapanã, Belém – PA, Brazil
3Departamento de Biocatalisis. ICP-CSIC, C/ Marie curie 2, Campus
The aim of this work was to produce esters with potential application as biodiesel via esterification with short-chain alcohols of free fatty acids (FFA) obtained from soybean and palm oils refining. Esterification reactions were catalyzed by dry lipase immobilized in fermented solids (DFS), obtained by Rhizomucor miehei grown on babassu cake by solid-state fermentation (SSF). A final conversion above 80% was attained using palm fatty acid distillate (PFAD) and soybean fatty acid distillate (SFAD) as raw materials in esterification reactions with ethanol. The biocatalyst could be reused 5 times in repeated 6h reactions after its washing with 95 % (v/v) ethanol or hexane (using SFAD as raw material). Moreover, the properties of the obtained fatty acid ethyl esters (FAEE)-enriched products were characterized. Both products were in accordance with several limits established by biodiesel-related standardized tests from Brazilian specifications including ester content (97.3% for the product obtained from SFAD), water content (180 mg.Kg-1 for the product obtained from PFAD), density (867.6 kg.(m3)-1for the product obtained from SFAD), viscosity kinematic (3.14-4.29 mm2.s-1), flash point (< 100°C), carbon residue (0.01 wt.%), ethanol (0.08-0.083 wt.%), free glycerol (<0.001 wt.%) and total glycerol (0.028-0.10 wt.%), monoglycerides (0.076-0.211 wt.%), diglycerides (0.0196-0.218 wt.%) and triglycerides (0.053-0.114 wt.%).
Keywords:fatty acid ethyl esters; biodiesel; palm fatty acid distillate; soybean fatty acid distillate; dry fermented solid; lipases.
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