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RevistaBiotechnology Research and Innovation

Support engineering: relation between development of new supports for immobilization of lipases and their applications

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Eliane Pereira Cipolattia, Evelin Andrade Manoela,b, Roberto Fernandez-Lafuentec, Denise Maria Guimarães Freirea
aLaboratório de Biotecnologia Microbiana, Departamento de Bioquímica, Instituto de
Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
bDepartamento de Biotecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Brazil.
cDepartamento de Biocatalisis, Instituto de Catalisis CSIC, Campus UAM–Cantoblanco, 28049 Madrid, Spain

The growing interest in processes with the use of immobilized lipases guides to the development of new supports. That way, the design and  characterization of new supports for lipase immobilization have been increasingly popular in literature. Efforts to obtain "the perfect support" (a not accomplished goal) are described in this paper. Obviously, the choice and development of a support is directly related to the process in which it will be used, considering different factors as the media where the immobilziwed enzyme will be used (whether aqueous, free or with solvents), potency of agitation, reactor configuration or substrates/products that will be involved. The present work discusses the use of some techniques of support synthesis in the case of core-shell particles, such as: miniemulsion, microemulsion emulsion, suspension, dispersion, the use of heterofunctional supports, whole-cell and processes of coimobilization. Some analytical tools for the investigation of enzyme immobilization are also presented, such as fourier transform infrared spectroscopy, as well as support charactristics that may be relevant for its final performance (e.g.,  specific surface area, particle diameter and particle size distribution and confocal laser scanning microscope). 

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