You can use the filter on the left to narrow the results
Previous
Type
All Books Papers
Title
Author
Keywords
ISBN
DOI Access
CSIC digital access
Search
Details
PublicationMolecules
Year2016
Volume21
Pages1577
International

Agarose and its derivatives as supports for enzyme immobilization

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimización de biocatalizadores y bioprocesos enzimáticos

Paolo Zucca 1, Roberto Fernandez-Lafuente 2, and Enrico Sanjust 1,*

1   Dipartimento di Scienze Biomediche, Università di Cagliari, Monserrato, Italy; pzucca@unica.it (P. Z.); sanjust@unica.it (E. S.)

2   Departamento de Biocatalisis, ICP-CSIC; C/ Marie Curie 2, Campus UAM-CSIC, Madrid 28049, Spain; rfl@icp.csic.es (R. F.-L.)

 

Agarose is a polysaccharide, coming from some seaweeds, with a quite particular structure allowing spontaneous gelation. Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and sharply hydrophilic. These features – that could be further improved by means of covalent cross-linking – render them particularly suitable for enzyme immobilization with a wide range of derivatization methods taking advantage from chemical modification of a fraction of the polymer hydroxyls. The main properties of the polymer are described here, followed by a review of cross-linking and derivatization methods. Some recent, innovative procedures to optimize the catalytic activity and operational stability of the obtained preparations are also described, together with multi-enzyme immobilized systems and the main guidelines to exploit their performances.

Keywords:enzymes; immobilization; stabilization; agar-agar; agarose; cross-linking; functionalization
logo de CSIC