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RevistaACS Catalysis

In situ determination of photobioproduction of H2 by In2S3-[NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough using only visible light

Autores:Cristina Tapia García, Sonia Zacarias , Ines A. C. Pereira , J. C. Conesa, Marcos Pita Martínez, Antonio López de Lacey
Grupos de investigación:BioElectroCatálisis, Catalizadores Nanoestructurados para Energía

 An interesting strategy for photocatalytic

production of hydrogen from water and sunlight is the

formation of a hybrid photocatalyst that combines an inorganic

semiconductor able to absorb in the visible light spectral range

with an enzymatic catalyst for reducing protons. In this work

we study how to optimize the interfacing of In2 S particles

with the soluble form of [NiFeSe] hydrogenase from

Desulfovibrio vulgaris  Hildenborough by means of its initial

H photoproduction rate. The kinetics of the photocatalytic

process was studied by membrane-inlet mass spectrometry, in

order to optimize the interaction between both components of

the hybrid photocatalyst. Membrane-inlet mass spectrometry allows measuring in the same experiment, for comparison, the rate

of H production by the photocatalyst hybrid directly in the aqueous solution in real time and the result of a standard assay of the

hydrogenase activity. An incubation period of 6 h with mild stirring of hydrogenase with In2 S particles was necessary for optimal

interaction of the enzyme molecules with the porous surface of the semiconductor. A turnover frequency of the NiFeSe

hydrogenase (TOFHase ) for H photobioproduction of 986 s was measured under the optimized conditions. This means that

the immobilized hydrogenase has a photocatalytic effi ciency for H generation which is 94% of that obtained in the standard

specifi c activity test of H production using reduced methyl viologen as an electron donor.

Palabras clave:Hydrogenase, Photocatalysis, Visible light, In2S3, hydrogen, Biocatalysis
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