ICP Instituto de Catalisis y Petroquímica

Cashew apple bagasse as a support for the immobilization of lipase B from Candida antarctica: Application to the chemoenzymatic production of (R)-Indanol
Ticiane C. de Souza^a, Thiago de S. Fonseca^b, Jessyca A. da Costa^a, Maria Valderez Ponte Rocha^a, Marcos Carlos de Mattos^b, Roberto Fernandez-Lafuente^c, Luciana R. B. Gonçalves^a Jose C. S. dos Santos^d*
^aDepartamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, CEP 60455-760, Fortaleza, CE, Brazil.
^bDepartamento de Química Orgânica e Inorgânica, Laboratório de Biotecnologia e Síntese Orgânica (LABS), Universidade Federal do Ceará, Campus do Pici,Caixa Postal 6044, 60455-970 Fortaleza, CE, Brazil.
^cICP-CSIC. Campus UAM-CSIC. Cantoblanco. 28049 Madrid. Spain.
^dInstituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, CEP 62785-000, Acarape, CE, Brazil.



Lipase B from Candida antarctica lipase expressed in Aspergillus niger has been covalently immobilized on cashew apple bagasse, and it stability and performance in the chemoenzymatic synthesis of (R)-Indano has been evaluated. The cashew apple bagasse (CAB) has been treated with alkaline hydrogen peroxide and activated with glycidol (GLY) to obtain glyoxyl groups, in addition to ethylenediamine and further activation with glutaraldehyde (GEG).The maximum loading of the support was 10 mg protein/g support, and its thermal and solvent stability was much higher than that of the soluble enzyme. The CAB-GEG preparation (with 95% yield of immobilization and 124% activity recovery) was used in the hydrolysis of ethyl rac-indanyl acetate  using an enzyme / substrate ratio of  2: 1 and a  pH value of 7.0 at 30°C for 24h. The conversion attained was 50% of conversion and e.e. of 99%. The reusability studies showed maintenance of conversion and e.e. during five cycles. The results suggest that the new CAB support may be an excellent alternative for lipase immobilization and stabilization.