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PublicationBioelectrochemistry
Year2015
Volume106
Pages335-342
International

Scaffold electrodes based on Thioctic acid-capped gold nanoparticles coordinated Alcohol Dehydrogenase and azure A films for high performance biosensor

Authors:Jose María Abad Pastor, Marcos Pita Martínez
Groups of research:BioElectroCatalysis
Nanometric size gold nanoparticles cappedwith thiotic acid are used to coordinatewith the Zn (II) present in the catalytic center of Alcohol Dehydrogenase (ADH). In combination with the NADH oxidation molecular catalyst Azure A, electrografted onto carbon screen-printed electrodes, they are used as scaffold electrodes for the construction of a very efficient ethanol biosensor. The final biosensing device exhibits a highly efficient ethanol oxidation with low overpotential of −0.25 V besides a very good analytical performance with a detection limit of 0.14 ± 0.01 μM and a stable response for more than one month.
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