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PublicationChemical Papers
Year2015
Volume69 (1)
Pages237-240.
International

Third-generation Oxygen Amperometric Biosensor Based on Trametes hirsuta Laccase Covalently Bound to Graphite Electrode

Authors:María Cristina Gutiérrez Sánchez, Sergey Shleev , Antonio López de Lacey, Marcos Pita Martínez
Groups of research:BioElectroCatalysis

The response of low-density graphite electrodes hosting Trametes hirsuta laccase in a direct electron

transfer regime is presented for real-time analysis of Oconcentrations. The use of contrasting

immobilisation methods developed for biocathodes affords good reproducibility and reliability of

the amperometric biosensor, which shows a limit of detection below 1 μM and a sensitivity slightly

higher than 60 nA cm−2 M−1

Keywords:laccase, Direct electron transfer, oxygen biosensor, bioelectrocatalysis
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