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Importance of the support properties for immobilization or purification of enzymes

Authors:José Cleiton Sousa dos Santos, Roberto Fernandez-Lafuente
Groups of research:Optimización de biocatalizadores y bioprocesos enzimáticos
Jose Cleiton S. dos Santosa,b, Oveimar Barbosac, Claudia Ortizd,  Angel Berenguer-Murciae, Rafael C. Rodrigues*,f, Roberto Fernandez-Lafuente*,a
a Departamento de Biocatalisis. ICP-CSIC. Campus UAM-CSIC. Cantoblanco. Madrid. Spain.
b Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, CEP 60455-760, Fortaleza, CE, Brazil.
c Grupo de investigación en productos naturales (GIPRONUT), Departamento de Química, Facultad de Ciencias, Universidad del Tolima, Ibagué, Colombia
d Escuela de Microbiología, UIS, Bucaramanga, Colombia.
e Instituto Universitario de Materiales, Departamento de Química Inorgánica, Universidad de Alicante, Campus de San Vicente del Raspeig, Ap. 99 - 03080 Alicante, Spain.
f  Biocatalysis and Enzyme Technology Lab, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, Porto Alegre, RS, Brazil.

Immobilization and purification of enzymes are usual requirements for their industrial use. Both purification and immobilization have a common factor: they use a solid activated support. Using a support for enzyme purification means having mild conditions for enzyme release and a selective enzyme-support interaction is interesting. Using a support for immobilization, enzyme desorption is a problem. The improvement of enzyme features via immobilization is a usual objective (e.g., stability, selectivity) Thus, a support designed for enzyme purification and a support designed for enzyme immobilization may differ significantly. In this review, we will try to focus the attention on the requirements of a support surface to produce the desired objectives. The ideal physical properties of the matrix, the properties of the introduced reactive groups, the best surface activation degree to reach the desired objective, and the properties of the reactive groups will be discussed.

Keywords:Enzyme stabilization, Enzyme immobilization, Enzyme purification, superficial density of reactive groups, multipoint attachment, one point attachment.
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