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Reconstitution of Respiratory Complex I on a Biomimetic Membrane Supported on Gold Electrodes

Autores:Gutiérrez-Sanz, Oscar, David Olea Duplan, Marcos Pita Martínez, Marisela Vélez Tirado, Antonio López de Lacey
Grupos de investigación:BioElectroCatalisis, Laboratorio de Biofuncionalización de Superficies
For the first time, respiratory complex I has been reconstituted
on an electrode preserving its structure and activity. Respiratory complex I is
a membrane-bound enzyme that has an essential function in cellular energy
production. It couples NADH:quinone oxidoreduction to translocation of
ions across the cellular (in prokaryotes) or mitochondrial membranes.
Therefore, complex I contributes to the establishment and maintenance of
the transmembrane difference of electrochemical potential required for
adenosine triphosphate synthesis, transport, and motility. Our new strategy
has been applied for reconstituting the bacterial complex I from Rhodothermus
marinus onto a biomimetic membrane supported on gold electrodes modified
with a thiol self-assembled monolayer (SAM). Atomic force microscopy and
faradaic impedance measurements give evidence of the biomimetic
construction, whereas electrochemical measurements show its functionality.
Both electron transfer and proton translocation by respiratory complex I were
monitored, simulating in vivo conditions.
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