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RevistaCurrent Organic Chemistry

Immobilization of proteins in poly-styrene-divinylbenzene matrices: functional properties and applications

Autores:Roberto Fernandez-Lafuente, José Cleiton Sousa dos Santos, Cristina Garcia-Galan
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Rafael C. Rodrigues1, Karel Hernandez2,3, Oveimar Barbosa2,4, Nazzoly Rueda4, Cristina Garcia-Galan2,  Jose C. S. dos Santos2,5,  Angel Berenguer-Murcia6, Roberto Fernandez-Lafuente2*
1 Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil
2 Departamento de Biocatalisis. ICP-CSIC. Campus UAM-CSIC. Cantoblanco. 28049 Madrid. Spain.
3 Permanent address: Biotransformation and Bioactive Molecules Group. Instituto de Química Avanzada de Cataluña-CSIC Jordi Girona 18-26, 08034. Barcelona (Spain)
4 Permanent address: Escuela de Química, Grupo de investigación en Bioquímica y Microbiología (GIBIM), Edificio Camilo Torres 210, Universidad Industrial de Santander, Bucaramanga, Colombia.
5 Permanent address: Departamento de Engenharia Química, Universidade Federal Do Ceará, Campus Do Pici, CEP 60455-760, Fortaleza, CE, Brazil.
6Instituto Universitario de Materiales, Departamento de Química Inorgánica, Universidad de Alicante, Campus de San Vicente del Raspeig, Ap. 99 - 03080 Alicante, Spain.

            Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact in enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.
Palabras clave:modulation of lipase properties, Hydrophobic supports, enzyme destabilization, interfacial activation of lipases, supported ionic liquids
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