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PublicationApplied Catalysis A: General
Pages 50-56

Optimization of ethyl ester production from olive and palm oils using mixtures of immobilized lipases

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimización de biocatalizadores y bioprocesos enzimáticos
Jakeline Kathiele Poppea, Carla Roberta Mattea, Maria do Carmo Ruaro Peralbab, Roberto Fernandez-Lafuentec, Rafael C. Rodriguesa, Marco Antônio Záchia Ayuba,*.
a Biotechnology, Bioprocess and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil;
b Department of Organic Chemistry, Institute of Chemistry, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil.
c Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, ZC 28049, Madrid, Spain

Although reactions of transesterification are generally catalyzed by one specific lipase preparation, the concept of “combi-lipase” could be better explored for the production of biodiesel, since oils are heterogeneous substrates. In this research we tested this concept by evaluating the enzymatic transesterification of olive and palm oils, two diverse fatty acid compositions, using stand alone or mixtures of three immobilized lipases as biocatalysts: Novozym 435 (CALB), Lipozyme TL-IM (TLL), and Lipozyme RM-IM (RML). For olive oil, the combination of 29.0% of TLL, 12.5% of RML, and 58.5% of CALB was the best combination, allowing for 95% conversion efficiency in 18 h of reaction, up from 50% for the best individual lipase (CALB). For palm oil, the best enzyme combination was 52.5% of TLL and 47.5% of RML, resulting in 80% of conversion of ethyl esters in 18 h, compared to only 44% when stand alone TLL was used. Repeated batches of reaction were carried out in order to test the operational stability of the combi-lipase systems, with results showing that they could be used for at least seven cycles keeping higher than 80% of their initial activities.
Keywords:ethyl esters; Thermomyces lanuginosus lipase; Rhizomucor miehei lipase; Candida antarctica lipase B; biodiesel; combi-lipase; enzyme reuse.
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