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PublicationMethods in Molecular Biology
Year2013
Volume1051
Pages149-163.
International

Tips for the functionalization of nanoparticles with antibodies

Authors:PUBLICACIONES
Groups of research:Enzymatic Engineering
Multiple antibody immobilization methodologies have been developed for several applications including affinity chromatography, immunosensing, and drug delivery. Most of them have been carried out without considering the orientation of the antigen binding site of the antibody, or after the chemical modification of the antibody. An efficient immobilization to improve the biological activity of the antibody is one of the key fundamental issues to pursue. A simple and effective methodology for well-oriented covalently immobilization of antibodies on nanoparticles is reported in this chapter.
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