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Publication Methods in Molecular Biology
Year2013
Volume1051
Pages 43-57.
International

Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports

Authors:PUBLICACIONES
Groups of research:Enzymatic Engineering
The immobilization of proteins on epoxy activated supports is discussed in this chapter. Immobilization on epoxy supports is carried out as a two-step mechanism: in the first step the adsorption of the protein is promoted and in the second step the intramolecular covalent linkage among epoxy groups and nucleophiles of the protein is produced. Based on this mechanism of the need of a first adsorption of the protein on the support, different epoxy supports are described. The different supports are able to immobilize proteins through different orientations being obtained catalysts with different properties of activity, stability, and selectivity.
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