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Publication (2013) Methods in Molecular Biology
Year2013
Volume1051
Pages73-88.
International

Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports

Authors:PUBLICACIONES
Groups of research:Enzymatic Engineering
Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contained the maximal concentration of glyoxyl groups to promote maximization of covalent immobilization and groups' capability to adsorb proteins by various mechanisms (e.g., ionic exchange, metal-chelate formation). Immobilization on various supports makes it possible to orientate and rigidify an enzyme in various regions of its surface. The use of different heterofunctional supports allowed for obtaining catalysts with different activity, stability, and selectivity properties.
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