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Revista (2013) Methods in Molecular Biology
Año2013
Volumen1051
Páginas73-88.
Internacional

Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports

Autores:PUBLICACIONES
Grupos de investigación:Ingeniería Enzimática
Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contained the maximal concentration of glyoxyl groups to promote maximization of covalent immobilization and groups' capability to adsorb proteins by various mechanisms (e.g., ionic exchange, metal-chelate formation). Immobilization on various supports makes it possible to orientate and rigidify an enzyme in various regions of its surface. The use of different heterofunctional supports allowed for obtaining catalysts with different activity, stability, and selectivity properties.
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