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RevistaJournal of Molecular catalysys B: Enzymatic
Año2014
Volumen105
Páginas18-25
Internacional

Comparison of the performance of commercial immobilized lipases in the synthesis of different flavor esters

Autores:Roberto Fernandez-Lafuente, Cristina Garcia-Galan
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Andréa B. Martins1, Alexandre M. da Silva1, Mirela F. Schein1, Cristina Garcia-Galan2, Marco A. Záchia Ayub1, Roberto Fernandez-Lafuente2*, Rafael C. Rodrigues1*
 
1Biotechnology, Bioprocess and Biocatalysis Group, Institute of Food Science and Technology, Federal University of Rio Grande do Sul State, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil;
2Department of Biocatalysis, ICP - CSIC. Campus UAM-CSIC. Cantoblanco, ZC 28049, Madrid, Spain.
In this work, it is compared the performance of three commercial lipase preparations (Novozym 435, Lipozyme TL-IM, and Lipozyme RM-IM) in the synthesis of flavor esters obtained by esterification of acetic, propionic, and butyric acids using ethanol, isopropyl alcohol, butanol, or pentanol. A comprehensive comparison was performed verifying activities of these 3 enzyme preparations versus the different couples of substrates, checking the obtained yields. In general, the longer the acid chain, the higher the reaction yields. Novozym 435 was the most efficient enzyme in most cases, and only Lipozyme RM-IM offered better results than Novozym 435 in the production of ethyl butyrate. Reactions with butyric acid showed the highest conversion rates using all biocatalysts. Using optimal substrates, the reactions catalyzed by the 3 enzymes were optimized using the response surface methodology, and the catalytic performance of the biocatalysts in repeated batches was assessed. After optimization, yields higher than 90 % were obtained for all three enzymes, but Lipozyme TL-IM needed 4-times more biocatalyst content than the other two preparations. Novozym 435 kept over 80 % of its activity when reused in 9 successive batches, whereas Lipozyme RM-IM can be reused 5 times and Lipozyme TL-IM only 3 times. In general, Novozym 435 showed to be more suitable for these reactions than the other two enzyme preparations
Palabras clave:Esterification; flavor esters; lipase; immobilized enzyme; enzyme reuse.
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