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PublicationRSC ADVANCES
Year2014
Volume4
Pages6863-6868
International

Combi-lipase for heterogeneous substrates: a new approach for hydrolysis of soybean oil using mixtures of biocatalysts

Authors:Roberto Fernandez-Lafuente
Groups of research:Optimización de biocatalizadores y bioprocesos enzimáticos
Joana S. Alves1, Nathália S. Vieira1, Alisson S. Cunha1, Alexandre M. Silva1, Marco A.
Záchia Ayub1, Roberto Fernandez-Lafuente2 and Rafael C. Rodrigues1,*
1Biotechnolgy, Bioprocess and Biocatalysis Group, Food Science and Technology Institute,
Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC
91501-970, Porto Alegre, RS, Brazil.
2Department of Biocatalysis, ICP - CSIC. Campus UAM-CSIC. Cantoblanco, ZC 28049,
Madrid, Spain.
Abstract
 It has been proposed the concept of combi-lipase biocatalyst. It is based on the
 combination of different lipases as biocatalysts in reactions using heterogeneous substrates.
 The hydrolysis of soybean oil was evaluated as a model substrate, and Novozym 435
 (CALB), Lipozyme TL-IM (TLL), and Lipozyme RM-IM (RML) were used as biocatalysts.
 Results showed that, although individually TLL was the most active enzyme, whereas CALB
 was the less active one, the combination of 80 % of RML and 20 % of CALB was the best
 biocatalyst. Reaction parameters were optimized, allowing to obtain more than 80 % of
 hydrolysis in 24 h using the combi-lipase, up from less than 50 % when any individual lipase.
 Reusability of the combi-lipase showed that it could be used for at least 15 cycles without any
significant decrease. The concept of combi-biocatalyst might be a useful technology for
 reactions including full modification of heterogeneous substrates.

Keywords:Oil hydrolysis; Novozym 435; Lipozyme TL-IM; Lipozyme TL-IM; soybean oil; 15 combi-lipase biocatalyst
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