Puede utilizar el filtro de búsqueda del panel izquierdo para acotar los resultados
Filtro
Tipo de publicación
Todos Libros Revistas
Título
Autor
Palabras clave
ISBN
Acceso DOI
Acceso digital CSIC
Buscar
Datos técnicos
RevistaJournal of Molecular catalysys B: Enzymatic
Año2014
Volumen100
Páginas59-67
Internacional

Preparation of Core-Shell Polymer Supports to immobilize lipase B from Candida antarctica. Effect of the support nature on catalytic properties

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Aline G. Cunhaa, Marina D. Bestetib, Evelin A. Manoelc, Angelo A. T. da Silva,d Rodrigo V. Almeida,a Alessandro B. C. Simas,d Roberto Fernandez-Lafuentee, José Carlos Pintob, * and
 Denise M. G. Freirea,*
a Programa de Pós-Graduação em Bioquímica/Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bloco A, 70 Andar, Rio de Janeiro, 21941-909 RJ, Brazil
bPrograma de Engenharia Química/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universitária, CP: 68502, Rio de Janeiro, 21941-972 RJ, Brazil
cPrograma de Pós-Graduação em Tecnologia de Processos Químicos e Bioquímicos/EQ, Universidade Federal do Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bloco E, 10 Andar, Rio de Janeiro, 21941-909 RJ, Brazil
dNúcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Cidade Universitária, Centro de Ciências da Saúde, Bloco H, Rio de Janeiro, 21941-902 RJ, Brazil
eDepartment of Biocatalysis, ICP - CSIC. Campus UAM-CSIC. Cantoblanco, ZC 28049, Madrid, Spain
Corel-shell supports have been prepared and utilized to immobilize lipase B from Candida antarctica. The hydrophobic nature of the supports permitted to immobilize the enzyme via interfacial activation at low ionic strength. Different supports were prepared having different hydrophobicity and crosslinking degree, and compared to the commercially available. Accurel MP 1000 (hydrophobic macroporous polymer of propylene) is a commercial support described as advantageous in different circumstances and it was used as comparative control in the process of immobilization.  The immobilized lipase preparations were evaluated in the hydrolysis of p-nitro-phenyl laurate and the esterification of oleic acid with ethanol. On the kinetic resolution of (±)-1,2-O-isopropylidene-3,6-di-O-benzyl-myo-inositol vinyl acetate was used as activated acyl donor. Results were very diverse, as the lipase properties may be easily tuned via immobilization, and some of the supports permitted to obtain activities even a two fold factor higher than the same amount of lipase immobilized in Accurel MP 1000. Moreover, in many instances, the loading of the support with enzyme produced reduced total activity in some reactions while not in other. This was explained by changes in the physical properties of the support surface that may alter the entry of substrates. Supports PS-co-DVB/PS-co-DVB 25% and PMMA-co-DVB/PMMA-co-DVB 25% presented very good features to immobilize CALB.

Palabras clave:Esterification, CALB, Lipase immobilization, interfacial activation, lipase modulation via immobilization, resolution of racemic mixtures
logo de CSIC