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PublicationBioelectrochemistry
Year2013
Volume94
Pages69-74
International

Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode

Authors:Marcos Pita Martínez, María Cristina Gutiérrez Sánchez, Miguel D Toscano , Sergey Shleev , Antonio López de Lacey
Groups of research:BioElectroCatalysis
Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6. ±. 1. μM with a linear range of 6-300. μM, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase.
Keywords:Direct electron transfer, Bilirubin oxidase, Gold nanoparticles, Biocatalysis, Biosensor
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