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RevistaFlavour and Fragrance Journal

Optimization of pineapple flavour synthesis by esterification catalysed by immobilized lipase from Rhizomucor miehei

Autores:Roberto Fernandez-Lafuente
Grupos de investigación:Optimización de biocatalizadores y bioprocesos enzimáticos
Lorenzoni, A.S.G., Graebin, N.G., Martins, A.B., Fernandez-Lafuente, R., Záchia Ayub, M.A., Rodrigues, R.C

AFFILIATIONS: Biocatalysis and Enzyme Technology Lab., Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box 15090, ZC 91501-970, Porto Alegre, RS, Brazil; 
Department of Biocatalysis, ICP-CSIC, Campus UAM-CSIC, Cantoblanco, Madrid 28049, Spain; 
Biochemical Engineering Lab (BiotecLab), Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500, P.O. Box15090, ZC, Porto Alegre, 91501-970, Brazil
The synthesis of pineapple flavour (butyl butyrate) catalysed by lipase from Rhizomucor miehei has been optimized using central composite design and response surface methodology. Initially, the best butyric acid concentration in the mixture was defined and found that 1 m butyric acid presented the highest initial reaction rate. The reaction parameters substrate molar ratio, enzyme content, and initial added water were evaluated in the central composite design with the reaction conversion yield as the dependent variable. The optimal conditions for butyl butyrate synthesis were found to be substrate molar ratio of 3.6:1 butanol:butyric acid; enzyme content of 6.5% of substrate mass fraction; added water 0.0% of substrate mass fraction. Under these conditions, over 90% of conversion was obtained in 16?h of reaction. Enzyme reuse was tested performing a treatment before each batch by washing the enzyme system with n-hexane, or simply reusing the biocatalyst in a new fresh reaction. Direct enzyme reuse caused a rapid decrease on the enzyme activity, while washings with n-hexane allowed the enzyme to be reused for six cycles keeping around 75% of its original activity.
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