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RevistaAdvanced Materials
Año2011
Volumen23
Páginas5275-5282
Internacional

Immobilized Biocatalysts: Novel Approaches and Tools for Binding Enzymes to Supports

Autores:Pamela Torres Salas, Alberto del Monte Martinez , Bessy Cutiño-Avila , Rodriguez-Colinas B., Miguel Alcalde Galeote, Antonio Ballesteros Olmo, Francisco José Plou Gasca
Grupos de investigación:Biocatálisis Aplicada

In the last decade, new trends for enzyme attachment to solid carriers have emerged in an attempt to rationalize the classical methods for enzyme immobilization. In silico analysis is becoming a powerful tool to predict the orientation of enzymes covalently attached to the carrier or the protein regions involved in adsorption to the support. Significantly, an array of algorithms has been established for the rational design of immobilized derivatives, which comprises both the protein size and the textural properties of the support. Ordered mesoporous materials open a challenging pathway to tailor immobilized enzymes with high volumetric activity and minimum lixiviation. In addition, fluorescence confocal microscopy is being successfully employed to understand the diffusional restrictions and the distribution of biomolecules within the support.

Palabras clave:Ordered mesoporous materials, enzymes, covalent immobilization, rational design of immobilized derivatives (RDID), fluorescence confocal microscopy
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