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Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods

María Cristina Gutiérrez Sánchez, Wenzhi Jia , Beyl Yvonne , Marcos Pita Martínez, Wolfgang Schuhmann , Antonio López de Lacey, Leonard Stoica
Electrochimica ActaThree immobilization protocols were investigated with respect to direct electron transfer between hierarchical carbon microfibers/carbon nanotubes composite material on graphite rod electrodes and Trametes hirsuta laccase. Immobilization was done by covalent binding of laccase to aminophenyl-modified electrodes via amide-bond formation with carboxylic acid residues or imino-bond formation with aldehyde groups introduced by oxidation of sugar residues of the enzyme’s glycosylation shell. Moreover, immobilization was achieved by adsorbing laccase to electrodes hydrophilized with pyrene-hexanoic acid. High current densities for biocatalytic oxygen reduction were obtained for all immobilization strategies. The formation of the imino bonds let to the binding of laccase in close to 100% direct electron transfer configuration and consequently to the highest oxygen reduction currents.
Three immobilization protocols were investigated with respect to direc...

Influence of H2S on ZrO2-based gasification gas clean-up catalysts: MeOH temperature-programmed reaction study

Søren Birk Rasmussen , Miguel A. Bañares
Applied Catalysis B: Environmental
Addition of H2S in the gasification gas stream has been found to impro...

Production of a α-L-Rhamnosidase from Aspergillus Terreus Using Citrus Solid Waste as Inducer for Application in Juice Industry

Rosa María Blanco Martín
Chemical Engineering Transactions
Naringinase is an enzyme complex consisting of a α-L-rhamnopyran...

Semisynthetic peptide-lipase conjugates for improved biotransformations

Chemical Communications,
An efficient chemoselective method for the creation of semisynthetic l...

Switchable Biofuel Cells Controlled by Biomolecular Computing Systems

Marcos Pita Martínez
International Journal of Unconventional Computing
Almost every existing device includes either a control or a self-regul...
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