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Dátos técnicos
Año2010
No. SolicitudPCT/ES11/070339
Alcance
StatusPCT
OEPM access
ES201030723
CSIC access
ES201030723

High redox potential lacases

Autores:Miguel Alcalde Galeote, Diana Maté Mate, Malena Valdivieso Ugarte, Layla Fernandez Torres
A Spanish Institute has developed a process to produce fungal laccases with high-redox potential by directed evolution and expression in yeast. A mutant laccase thus produced shows high activity (6-fold higher than that of similar laccases) and stability to the presence of organic solvents, temperature (T50=73ºC) and pH (pH=3-9) and is secreted at 8 mg/ml of broth.
Exploitation of this OB-1 laccase and other obtainable with this procedure is offered under a patent licence.
Palabras clave:lacasas, evolucion dirigida, enzimas
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