Applied Biocatalysis Group
It's all possible with enzymes
OUR BOOK CHAPTERS and REVIEWS on PREBIOTICS SYNTHESIS
“Efficient fructooligosaccharide synthesis with a fructosyltransferase from Aspergillus aculeatus”. F.J. Plou, M. Alcalde, I. Ghazi, L. Fernández-Arrojo and A. Ballesteros. In: “Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions“ (W.D. Fessner, T. Anthonsen, Eds.), Wiley Interscience, New York, ISBN-10: 3-527-32071-7, pp. 153-171 (2009). More
“Application of glycosidases and transglycosidases for the synthesis of oligosaccharides”. F.J. Plou, A. Gómez de Segura and A. Ballesteros. in: “Industrial Enzymes: Structure, Function and Applications” (J. Polaina and A.P. MacCabe, Eds.), Springer, New York, ISBN 978-1-4020-5376-4, p. 141-157 (2007). More
“Enzymatic synthesis of sugar esters and oligosaccharides from renewable resources”. A. Ballesteros, F.J. Plou, M. Alcalde, M. Ferrer, H. García-Arellano, D. Reyes-Duarte and I. Ghazi. In: Biocatalysis in the Pharmaceutical and Biotechnological Industries (R. Patel, Ed.), CRC Press, New York, ISBN 978-0-8493-3732-1, p. 465-490 (2006). More
“Glucosyltransferases acting on starch or sucrose for the synthesis of oligosaccharides”. F.J. Plou, M.T. Martin, A. Gómez de Segura, M. Alcalde and A. Ballesteros. Canadian Journal of Chemistry 80, 743-752 (2002). doi:10.1139/V02-104
PUBLICATIONS
Enzymatic synthesis of oligosaccharides (prebiotics)
Fructooligosaccharides (FOS):
| “Molecular and biochemical characterization of a beta-fructofuranosidase from Xanthophyllomyces dendrorhous”. D. Linde, I. Macias, L. Fernández-Arrojo, F.J. Plou and M. Fernández-Lobato. Applied and Environmental Microbiology, 75, 1065-1073 (2009). | |
| doi:10.1128/AEM.02061-08 | |
| “Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose”. M. Alvaro-Benito, M. de Abreu, L. Fernandez-Arrojo, F.J. Plou, J. Jiménez-Barbero, A. Ballesteros, J. Polaina and M. Fernández-Lobato. Journal of Biotechnology, 132, 75-81 (2007). | |
| doi:10.1016/j.jbiotec.2007.07.939 | |
| “On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics”. L. Fernández-Arrojo, M. Alvaro, I. Ghazi, M. de Abreu, D. Linde, P. Gutiérrez-Alonso, M. Alcalde, J. Jimenez-Barbero, A. Jimenez, A. Ballesteros, M. Fernández-Lobato and F.J. Plou. Journal of Biotechnology, 131S, S107 (2007). | |
| doi:10.1016/j.jbiotec.2007.07.184 |  |
| “Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus”. I. Ghazi, L. Fernández-Arrojo, H. García-Arellano, M. Ferrer, F.J. Plou and A. Ballesteros. Journal of Biotechnology, 128, 204-211 (2007). | |
| doi:10.1016/j.jbiotec.2006.09.017 | |
| “Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides”. I. Ghazi, L. Fernández-Arrojo, A. Gómez de Segura, M. Alcalde, F.J. Plou and A. Ballesteros. Journal of Agricultural and Food Chemistry, 54, 2964-2968 (2006) | |
| doi:10.1021/jf053023b | |
| “Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides”. I. Ghazi, A. Gómez de Segura, L. Fernández-Arrojo, M. Alcalde, M. Yates, M.L. Rojas-Cervantes, F.J. Plou and A. Ballesteros. Journal of Molecular Catalysis B: Enzymatic, 35, 19-27 (2005). | |
| doi: 10.1016/j.molcatb.2005.04.013 | |
Isomaltooligosaccharides (IMOS):
| “Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous”. L. Fernández-Arrojo, D. Marín, A. Gómez de Segura, D. Linde, M. Alcalde, P. Gutiérrez-Alonso, I. Ghazi, F.J. Plou, M. Fernández-Lobato and A. Ballesteros. Process Biochemistry, 42, 1530-1536 (2007). | |
| doi:10.1016/j.procbio.2007.08.007 | |
| “Synthesis of methyl alpha-D-glucooligosaccharides by entrapped dextransucrase from Leuconostoc mesenteroides B-1299”. A. Gómez de Segura, M. Alcalde, M. Bernabé, A. Ballesteros and F.J. Plou. Journal of Biotechnology, 124, 439-445 (2006) | |
| doi:10.1016/j.jbiotec.2005.12.031 | |
| “Effect of acceptor reaction on dextran production using native and encapsulated dextransucrases from Leuconostoc mesenteroides B-512F and B-1299”. A. Gómez de Segura, M. Alcalde, N. López-Cortés and F.J. Plou. Food Technology and Biotechnology, 42, 337-342 (2004). | |
| Access | |
“Immobilization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F on Eupergit C supports”. A.Gómez de Segura, M. Alcalde M. Yates, M.L. Rojas-Cervantes, N. López-Cortés, A. Ballesteros and F.J. Plou. Biotechnology Progress, 20, 1414-1420 (2004). |
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| doi:10.1021/bp0400083 | |
Coupling sugar:
“Synthesis of maltooligosyl fructofuranosides catalysed by immobilized cyclodextrin glucosyltransferase using starch as donor”. M.T. Martín, M.A. Cruces, M. Alcalde, F.J. Plou, M. Bernabé and A. Ballesteros. Tetrahedron, 60, 529-534 (2004). |
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| doi:10.1016/j.tet.2003.10.113 | |
Maltooligosaccharides (MOS):
“Chemical modification of carboxylic residues in a cyclodextrin glucanotransferase and its implication in the hydrolysis/transglycosylation ratio of the alpha-amylase family”. M. Alcalde, F.J. Plou, M. Pérez-Boada, H. García–Arellano, I. Valdés, E. Méndez and A. Ballesteros. Journal Molecular of Catalysis B: Enzymatic, 26, 57-67 (2003). |
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| doi:10.1016/S1381-1177(03)00166-8 | |