It's all possible with enzymes
“Molecular and biochemical characterization of a beta- fructofuranosidase from X. dendrorhous”. D. Linde, I. Macias, L. Fernández-Arrojo, F.J. Plou and M. Fernández-Lobato. Applied and Environmental Microbiology (2009). more
“Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers”. P. Torres, D. Reyes-Duarte, N. López-Cortés, M. Ferrer, A. Ballesteros and F.J. Plou. Process Biochemistry 42, 145-153 (2008). more
“Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra”. Zumarraga, M., Camarero, S., Martinez-Arias, A., Ballesteros, A., Plou, F.J. and Alcalde, M. Proteins: Structure, function and bioinformatics. 71: 250-260 (2008). more


PUBLICATIONS
Directed evolution
“Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra". M. Zumárraga, S. Camarero, S. Shleev, A. Martínez-Arias, A. Ballesteros, F.J. Plou, and M. Alcalde. Proteins, 71, 250-260 (2008).
Enzymatic synthesis of oligosaccharides (prebiotics)
“Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous”. L. Fernández-Arrojo, D. Marín, A. Gómez de Segura, D. Linde, M. Alcalde, P. Gutiérrez-Alonso, I. Ghazi, F.J. Plou, M. Fernández-Lobato and A. Ballesteros. Process Biochemistry, 42, 1530-1536 (2007)
Enzymatic modification of antioxidants
“Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers”. P. Torres, D. Reyes-Duarte, N. López-Cortés, M. Ferrer, A. Ballesteros and F.J. Plou. Process Biochemistry 42, 145-153 (2008).
Bioremediation with enzymes
“Bioremediation of polycyclic aromatic hydrocarbons by fungal laccases engineered MtLT2 by directed evolution”. M. Zumárraga, F.J. Plou, H. García-Arellano, A. Ballesteros and M. Alcalde. Biocatalysis and Biotransformation, 25, 219-228 (2007).
Enzyme immobilization
“Characterization and application of a sterol esterase immobilized on polyacrylate epoxy-activated carriers (Dilbeads)”. P. Torres, et al. Catalysis Communications, 9, 539-545 (2008).
Enzymatic synthesis of carbohydrate esters
“Factors affecting productivity in the lipase-catalysed synthesis of sucrose palmitate”. D. Reyes-Duarte, N. López-Cortés, M. Ferrer, F.J. Plou, and A. Ballesteros. Biocatalysis & Biotransformation, 23, 19-27 (2005).