Applied Biocatalysis Group
"The power of enzymes"
Latest Publications
"Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast". E. García-Ruiz, D. Maté, A. Ballesteros, A.T. Martínez and M. Alcalde. Microbial Cell Factories 9:17 (2010). doi:10.1186/1475-2859-9-17
“Regioselective Lipase-Catalyzed Synthesis of 3-O-Acyl Derivatives of Resveratrol and Study of Their Antioxidant Properties”. P. Torres, A. Poveda, J. Jimenez-Barbero, A. Ballesteros, and F.J. Plou. Journal of Agricultural and Food Chemistry,58, 807-813 (2010) doi: 10.1021/jf903210q
“Biochemical characterization of a beta-fructofuranosidase from Rhodotorula dairenensis with transfructosylating activity”. P. Gutiérrez-Alonso, L. Fernández-Arrojo, F.J. Plou and M. Fernández-Lobato. FEMS Yeast Research 9, 768-773 (2009) doi: 10.1111/j.1567-1364.2009.00526.x
PUBLICATIONS
Directed evolution
“Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra". M. Zumárraga, S. Camarero, S. Shleev, A. Martínez-Arias, A. Ballesteros, F.J. Plou, and M. Alcalde. Proteins, 71, 250-260 (2008).
Enzymatic synthesis of oligosaccharides (prebiotics)
“Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous”. L. Fernández-Arrojo, D. Marín, A. Gómez de Segura, D. Linde, M. Alcalde, P. Gutiérrez-Alonso, I. Ghazi, F.J. Plou, M. Fernández-Lobato and A. Ballesteros. Process Biochemistry, 42, 1530-1536 (2007)
Enzymatic modification of antioxidants
“Acetylation of vitamin E by Candida antarctica lipase B immobilized on different carriers”. P. Torres, D. Reyes-Duarte, N. López-Cortés, M. Ferrer, A. Ballesteros and F.J. Plou. Process Biochemistry 42, 145-153 (2008).
Bioremediation with enzymes
“Bioremediation of polycyclic aromatic hydrocarbons by fungal laccases engineered MtLT2 by directed evolution”. M. Zumárraga, F.J. Plou, H. García-Arellano, A. Ballesteros and M. Alcalde. Biocatalysis and Biotransformation, 25, 219-228 (2007).
Enzyme immobilization
“Characterization and application of a sterol esterase immobilized on polyacrylate epoxy-activated carriers (Dilbeads)”. P. Torres, et al. Catalysis Communications, 9, 539-545 (2008).
Enzymatic synthesis of carbohydrate esters
“Factors affecting productivity in the lipase-catalysed synthesis of sucrose palmitate”. D. Reyes-Duarte, N. López-Cortés, M. Ferrer, F.J. Plou, and A. Ballesteros. Biocatalysis & Biotransformation, 23, 19-27 (2005).