Applied Biocatalysis Group
"The power of enzymes"
The Applied Biocatalysis Group in Madrid has experience in the techniques that are relevant to our research:
- Different methodologies for the determination of enzyme activity, e.g. fluorimetric, spectrophotometric and pH static techniques, HPLC.
- Molecular biology techniques.
- High-throughput screening methods
- Methodology for the immobilization/stabilization of proteins
- Chromatographic techniques for purifying and characterizing native and modified enzymes (gel filtration, electrophoresis, FPLC, isoelectrofocusing) and for following the progress of the enzymatic reaction (TLC, HPLC, gas-liquid chromatography)
- Nuclear magnetic resonance for identifying reaction products
In addition, the following techniques are available at the Institute:
- Atomic Emission Spectroscopy (ICP) (Perkin-Elmer)
- Atomic Absorption Spectroscopy (Perkin-Elmer)
- FTIR Spectroscopy (Nicolet)
- UV-VIS Spectroscopy / Diffuse Reflectance (Shimadzu)
- N2 Isotherm. B.E.T. Surface (Micromeritics)
- Mercury Porosimetry (Micromeritics)
- Flow Chemisorption (Micromeritics)
- X-Ray Diffraction (polycrystalline) (Seifert)
- Fluorimetry (Perkin-Elmer)
- FT-Raman (Bruker)
- RSE (Bruker)
- XPS (VG)
Equipment
Chromatography
Ternary HPLC (Varian) with high-throughput autosampler (Merck). Photodiode-array detector (Varian) put in series with an evaporative light-scattering detector ELDS-2000ES (Alltech) controlled by software Varian Star 4.0. Applications: analysis of antioxidants, lipids, sugar esters, etc. |
Semipreparative HPLC Delta 600 (Waters) with autosampler. UV-VIS detector (Spectra-Physics) put in series with a light-scattering detector DL31 (Eurosep) controlled by software Millennium (Waters). Applications: analysis of carbohydrates, purification of carbohydrates and proteins, etc. |
Anionic chromatography with pulse-amperometric detector HPAEC-PAD (Dionex). Applications: analysis of polysaccharides, oligosaccharides and monosaccharides, etc. |
Semipreparative HPLC quaternary pump 600E (Waters) with autosampler ProStar (Varian). Refraction index detector (Waters) put in series with a photodiode-array detector (Varian) controlled by software Varian Star 4.0. Applications: analysis of pharmaceuticals, purification of proteins, etc. |
Microplate reader VERSAMax (Molecular Devices) |
Microplate reader SpectraMax Plus 384 (Molecular Devices) |
UV-VIS spectrophotometer UV-1800 (Shimadzu) |
Automatic titrator DL50 (Mettler) |
Enzyme purification and characterization
Enzyme purifier AKTA (FPLC) from GE Healthcare |
Devices for protein characterization (electrophoresis, isoelectric point determination, concentration, etc.) |
High-throughput screening
Liquid handlers Quadra 96 (Tomtec) |
Multidrop (Thermo) |
Centrifuge for microplates (Sorvall) |
Molecular Biology
Thermocyclers (BioRad) |
Vertical laminar flow clean bench, Mini-V/PCR (Telstar) |
Digitalization camera (BioRad) |
Trans-Blot (Bio-Rad) |
Shakers
Environmental shaker (Biosan) --------- Humidity shaker Minitron (Infors)
Orbital shaker (Stuart Scientific)
Others
Water activity (Novasina)
Device for sample degassification
Karl-Fisher DL31 (Mettler) for determination of water concentration
Rotary evaporator RE-121 (Büchi)